Abstract Search

ea0035p946 | Steroid metabolism and action | ECE2014

Measurement of testosterone, androstenedione and dehydroepiandrosterone (DHEA) serum levels using isotope-dilution liquid-chromatography tandem mass spectrometry (ID-LC-MS/MS)

Buttler Rahel M , Martens Frans , Ackermans Mariette T , Blankenstein Marinus A , Heijboer Annemieke C

The adrenal and gonadal androgens testosterone, androstenedione and dehydroepiandrosterone (DHEA) play an important role in sexual development and fertility as well as in several other processes. We developed a method to assess serum testosterone, androstenedione and DHEA levels in one run using isotope-dilution liquid-chromatography tandem mass spectrometry (ID-LC-MS/MS). Sample preparation consisted of addition of internal standards (13C3-testosterone, ...

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ePoster

ea0070aep14 | Adrenal and Cardiovascular Endocrinology | ECE2020

Comparability of steroid hormone measurement among 9 European laboratories using liquid chromatography-tandem mass spectrometry (LC-MS/MS): Impact of the blood derivative and of calibration

Fanelli Flaminia , Mezzullo Marco , Nardi Elena , Cantù Marco , Lindner Johanna M , Peitzsch Mirko , Hawley James M , Bruce Stephen J , Heijboer Annemieke C , Ackermans Mariette T , Van den Ouweland Jody , Koeppl Daniel , Eisenhofer Graeme , Rauh Manfred , Keevil Brian , Vogeser Michael , Pagotto Uberto

Background: LC-MS/MS is recommended for accurately measuring circulating steroids. However, laboratories using LC-MS/MS adopt various pre-analytical and analytical strategies, and little is known about comparability of results. Whether different blood derivatives impact LC-MS/MS measurements is unclear, and the benefit of unifying the calibration system among laboratories was not tested.Aim: Comparisons of LC-MS/MS measurements of circulating aldosterone...

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Endocrine Abstracts

Measurement of testosterone, androstenedione and dehydroepiandrosterone (DHEA) serum levels using isotope-dilution liquid-chromatography tandem mass spectrometry (ID-LC-MS/MS)

Comparability of steroid hormone measurement among 9 European laboratories using liquid chromatography-tandem mass spectrometry (LC-MS/MS): Impact of the blood derivative and of calibration

BiosciAbstracts